Uukuniemi virus infection causes a pervasive remodelling of the RNA-binding proteome in tick cells

• DOI: 10.1101/2024.09.06.611624
• Publication Date: 2024-09-07T05:35:18Z
• Authors: Alexandra Wilson; Wael Kamel; Kelsey Davies; Zaydah R. De Laurent; Rozeena Arif; Lesley Bell-Sakyi; Douglas Lamont; Yana Demyanenko; Marko Noerenberg; Alain Kohl; Shabaz Mohammed; Alfredo Castello; Benjamin Brennan
• Keywords: Proteome

Abstract RNA-binding proteins (RBPs) are pivotal for the viral lifecycle, mediating key host-virus interactions that promote or repress virus infection. While these have been largely studied in vertebrate host, no comprehensive analyses of protein-RNA occurring cells derived from infected vector done to date, particularly ticks. Here we systematically identify responses proteome (RBPome) infection with a prototype bunyavirus (Uukuniemi virus; rUUKV) tick and discovered changes activity 283 proteins. In an orthogonal approach, analysed ribonucleoprotein by immunoprecipitation UUKV nucleocapsid (NCAP) cells. We found cell RBPs regulated associate N complexes, suggesting functional connection confirmed experimentally impact infection, TOP3B showing putative effect on particle production. Our data thus reveals first interaction map determined Research highlights RNAs interact nearly three hundred enrichment NCAP interactors within upregulated RIC direct involvement RNA processing Developed robust methodology silence gene expression cultures The complex facilitates efficient packaging virions.