Integrated single cell analysis of blood and cerebrospinal fluid leukocytes in multiple sclerosis

• DOI: 10.1101/403527
• Publication Date: 2018-08-29T14:59:27Z
• Authors: David Schafflick; Chenling A. Xu; Maike Hartlehnert; Michael Cole; Tobias Lautwein; Andreas Schulte-M...; Jolien Wolbert; Michael Heming; Sven G. Meuth; Tanja Kuhlmann; Catharina C. Gross; Heinz Wiendl; Nir Yosef; Gerd Meyer zu Horste
• Keywords: Central Nervous System; 0301 basic medicine; Encephalomyelitis, Autoimmune, Experimental; Multiple Sclerosis; Helper-Inducer; T-Lymphocytes; Science; Neurodegenerative; Autoimmune Disease; Article; Experimental; Mice; 03 medical and health sciences; T-Lymphocyte Subsets; Genetics; Leukocytes; 2.1 Biological and endogenous factors; Animals; Humans; Aetiology; Encephalomyelitis; Cerebrospinal Fluid; B-Lymphocytes; 0303 health sciences; Blood Cells; Gene Expression Profiling; Human Genome; Q; Neurosciences; T-Lymphocytes, Helper-Inducer; Brain Disorders; 3. Good health; Phenotype; Neurological; Single-Cell Analysis; Autoimmune

Summary Cerebrospinal fluid (CSF) protects the central nervous system (CNS) and analyzing CSF aids diagnosis of CNS diseases, but our understanding leukocytes remains superficial. Here, we firstly provide a transcriptional map single in compared to blood. Leukocyte composition transcriptome were compartment-specific with CSF-enrichment myeloid dendritic cells border-associated phenotype monocytes. We secondly tested how multiple sclerosis (MS) - an autoimmune disease affected both compartments. MS increased diversity blood, while it preferentially cell type CSF. In addition known expansion B lineage cells, identified increase cytotoxic-phenotype follicular T helper (TFH) mice, TFH accordingly promoted infiltration into severity animal models. Immune mechanisms are thus highly compartmentalized indicate local T/B interaction.