A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program.

• DOI: 10.1101/gad.3.5.628
• Publication Date: 2007-06-05T21:15:46Z
• Authors: D G Edmondson; E N Olson
• Keywords: Chloramphenicol O-Acetyltransferase; 0301 basic medicine; 0303 health sciences; Base Sequence; Muscles; Genetic Vectors; Molecular Sequence Data; Muscle Proteins; Nuclear Proteins; DNA; Blotting, Northern; Muscle Development; Phosphoproteins; Cell Line; Blotting, Southern; 03 medical and health sciences; Enhancer Elements, Genetic; Gene Expression Regulation; Proto-Oncogenes; RNA; Myogenin; Amino Acid Sequence; MyoD Protein

MyoD1 is a nuclear phosphoprotein that expressed in skeletal muscle vivo and certain cell lines vitro; it has been shown to convert fibroblasts myoblasts through mechanism requiring domain with homology the myc family of proteins. The BC3H1 line expresses muscle-specific genes upon exposure mitogen-deficient medium, but does not express at detectable levels. To determine whether cells may regulatory functionally related MyoD1, cDNA library prepared from differentiated myocytes, was screened reduced stringency region shares c-myc. From this screen, identified encodes major open reading frame 72% basic MyoD1. mRNA encoded by MyoD1-related gene myocytes vitro undetectable cardiac or smooth muscle, nonmuscle tissues, nonmyogenic types. During myogenesis, accumulates several hours prior other mRNAs therefore represents an early molecular marker for entry into differentiation pathway. Transient transfection 10T1/2 3T3 sufficient induce myosin heavy-chain expression activate reporter under transcriptional control creatine kinase 5' enhancer, which functions only myocytes. Expression stably transfected also leads fusion medium. Thus, product program substitute developmental situations. Together, these results suggest existence myogenic share conserved motif