Genomewide Trapping of Genes that Encode Secreted and Transmembrane Proteins Repressed by Oncogenic Signaling

• DOI: 10.1101/gr.202601
• Publication Date: 2018-09-18T16:23:37Z
• Authors: Mathias Gebauer; Harald von Melchner; Thomas Beckers
• Keywords: Coding region

A retroviral gene trap containing a human CD2 cell surface antigen/neomycin–phosphotransferase fusion in the U3 region of its LTR (U3Ceo) was used to screen mammalian genome for genes encoding secreted and/or transmembrane proteins that are repressed by oncogenic transformation. From an integration library consisting cells transformable insulin-like growth factor 1 (IGF-1), collection neomycin resistant (Neo R ) clones obtained; 86% also expressed antigen. Molecular analysis random sample Neo revealed U3Ceo preferentially disrupted coding and proteins. In each case, signal sequence endogenous fused in-frame CD2/neomycin–phosphotransferase reporter due cryptic splice acceptor site embedded cDNA. When transformed IGF-1 selected against expression, integrations were obtained six randomly chosen that, captured from involved transformation metastatic spread.