The Arabidopsis Purple Acid Phosphatase AtPAP10 Is Predominantly Associated with the Root Surface and Plays an Important Role in Plant Tolerance to Phosphate Limitation

• DOI: 10.1104/pp.111.183723
• Publication Date: 2011-09-23T08:00:11Z
• Authors: Liangsheng Wang; Zheng Li; Weiqiang Qian; Wanli Guo; Xiang Gao; Lingling Huang; Han Wang; Huifen Zhu; Jia-Wei Wu; Daowen Wang; Dong Liu
• Keywords: 0301 basic medicine; 2. Zero hunger; Arabidopsis Proteins; Surface Properties; Acid Phosphatase; Green Fluorescent Proteins; Molecular Sequence Data; Arabidopsis; Adaptation, Physiological; Plant Roots; Phosphates; 03 medical and health sciences; Phenotype; Gene Expression Regulation, Plant; Mutation; Amino Acid Sequence; Cloning, Molecular; Glycoproteins

Abstract Induction of secreted acid phosphatase (APase) is a universal response higher plants to phosphate (Pi) limitation. These enzymes are thought scavenge Pi from organophosphate compounds in the rhizosphere and thus increase availability when deficient. The tight association APase with root surface may make more efficient utilization soil around tissues, which present forms. To date, however, no systematic molecular, biochemical, functional studies have been reported for any starvation-induced APases that associated after secretion. In this work, using genetic molecular approaches, we identified Arabidopsis (Arabidopsis thaliana) Purple Acid Phosphatase10 (AtPAP10) as predominantly surface. AtPAP10 protein has activity against variety substrates. Expression specifically induced by limitation at both transcriptional posttranscriptional levels. Functional analyses multiple atpap10 mutant alleles overexpressing lines indicated plays an important role plant tolerance Genetic manipulation expression provide effective means engineering new crops increased deprivation.