Isolation and functional analysis of theKlPDR16gene

• DOI: 10.1111/1567-1364.12102
• Publication Date: 2013-10-12T01:40:32Z
• Authors: Eduard Goffa; Zuzana Balazfyova; Nora Toth Hervay; Zuzana Simova; Maria Balazova; Peter Griac; Yvetta Gbelska
• Keywords: Kluyveromyces lactis; Oligomycin; Efflux; Rhodamine 123; Ethidium bromide; Rhodamine 6G; Ergosterol

The fight against multidrug-resistant pathogens requires an understanding of the underlying cellular mechanisms. In this work, we isolate and characterize one multidrug resistance determinants in Kluyveromyces lactis, KlPDR16 gene. We show that KlPdr16p (345 aa), which belongs to KlPdr1p regulon, is a functional homologue Saccharomyces cerevisiae Pdr16p. Deletion resulted hypersensitivity K. lactis cells antifungal azoles, oligomycin, rhodamine 6G, 4-nitroquinoline-N-oxide alkali metal cations. Klpdr16∆ mutation led decreased content ergosterol whole-cell extract. spite mutant 6G transcript level KlPDR5 gene efflux was same as parental strain. Increased accumulation indicates limits rate passive drug diffusion across membrane, without affecting glucose-induced export. results obtained KlPDR16, similar its orthologues other yeast species, influences into cell.