The c‐Myc‐regulated miR‐17‐92 cluster mediates ATRA‐induced APL cell differentiation

• DOI: 10.1111/ajco.13225
• Publication Date: 2019-07-02T02:09:11Z
• Authors: Xibao Yu; Yanyun Hu; Yifan Wu; Chunsheng Fang; Jing Lai; Shaohua Chen; Yangqiu Li; Chengwu Zeng; Yixin Zeng
• Keywords: Cluster of differentiation; Promyelocytic leukemia protein

Abstract Background Despite advances in the treatment of acute promyelocytic leukemia (APL) with all‐ trans ‐retinoic acid (ATRA), its underlying mechanism has not been fully elucidated. The oncogenic microRNA cluster miR‐17‐92 modulates multiple cellular processes, including survival, proliferation, and apoptosis. However, role regulation yet documented for APL. Methods We analyzed expression APL samples cell lines by qRT‐PCR. c‐Myc was measured western blot. Cell differentiation assessed measuring surface CD11b antigen flow cytometry analysis. Results observed that upregulated compared healthy donors. Furthermore, we demonstrated expressions are markedly suppressed during ATRA‐induced NB4 differentiation. Importantly, also is directly regulated granulocytic cells. Finally, overexpression miR‐17‐5p blocks Conclusions report abnormal cells, which responsible block blast cells In addition, identified as a target gene