Physalin B inhibits cell proliferation and induces apoptosis in undifferentiated human gastric cancer HGC‐27 cells

• DOI: 10.1111/ajco.13593
• Publication Date: 2021-06-23T17:24:13Z
• Authors: Chunsheng Fang; Cunte Chen; Yanjun Yang; Kehan Li; Rili Gao; Dandan Xu; Youxue Huang; Zheng Chen; Zhuandi Liu; Shaohua Chen; Xibao Yu; Yangqiu Li; Chengwu Zeng
• Keywords: Cyclin-dependent kinase 6; Viability assay; CDK inhibitor; Cyclin D

Abstract Background Physalin B (PB) from Physalis angulata L . (Solanaceae) is a naturally occurring secosteroid with multiple biological activities, including anti‐inflammatory and anticancer activity. However, PB's effects mechanisms in human gastric cancer (GC) cells are not well characterized. Methods The undifferentiated GC cell line HGC‐27 semi‐differentiated SGC‐7901 were treated PB. Cell counting kit‐8 (CCK‐8) colony formation assays performed to evaluate viability. Apoptosis the cycle assessed by Annexin V/PI PI/RNase DNA staining assays, respectively, Western blotting was used expression of protein. Results PB significantly inhibited proliferation dose‐ time‐dependent manner. Moreover, induced G0/G1 arrest caspase‐dependent apoptosis cells. Cleaved caspases 8, 3, 7, poly(ADP)‐ribose polymerase (PARP), cyclin‐dependent kinase (CDK) inhibitor p‐Chk2 cells, while cycle‐related proteins cyclin D1, D3, CDK4, CDK6, E, phosphorylated retinoblastoma tumor suppressor protein (p‐Rb) downregulated dose‐dependent Conclusions inhibits via induces suggesting that might be novel effective agent for therapy.