A Comprehensive Analysis of the Cellular and EBV-Specific MicroRNAome in Primary CNS PTLD Identifies Different Patterns Among EBV-Associated Tumors

• DOI: 10.1111/ajt.12858
• Publication Date: 2014-08-04T20:57:29Z
• Authors: S.E.K. Fink; M.K. Gandhi; J.P. Nourse; C. Keane; K. Jones; P. Crooks; K. Jöhrens; A. Korfel; H. Schmidt; S. Neumann; A. Tiede; U. Jäger; U. Dührsen; R. Neuhaus; M. Dreyling; K. Borchert; T. Südhoff; H. Riess; I. Anagnostopoulos; R.U. Trappe
• Keywords: Lytic cycle; Epstein–Barr virus infection; Lymphoproliferative Disorders

Primary central nervous system (pCNS) posttransplant lymphoproliferative disorder (PTLD) is a complication of solid organ transplantation characterized by poor outcome. In contrast to systemic PTLD, Epstein-Barr virus (EBV)-association pCNS PTLD almost universal, yet viral and cellular data are limited. To identify differences in the pattern EBV-association we analyzed expression latent lytic EBV transcripts microRNAome nine (eight EBV-associated) 16 samples EBV-associated). Notably although 15/16 EBV-associated exhibited type III latency pattern, were also strongly expressed. Members ebv-miR-BHRF1 ebv-miR-BART clusters expressed virtually all samples. There 28 microRNAs differentially between PTLD. lower hsa-miR-199a-5p/3p hsa-miR-143/145 (implicated nuclear factor kappa beta c-myc signaling) as compared Unsupervised nonhierarchical clustering distinguished non-EBV-associated from identified separate group that displayed reduced levels B cell lymphoma associated oncomiRs such hsa-miR-155, -21, -221 hsa-miR-17-92 cluster. has major impact on microRNA