miR‐146a enhances regulatory T‐cell differentiation and function in allergic rhinitis by targeting STAT5b

• DOI: 10.1111/all.15163
• Publication Date: 2021-10-30T18:14:54Z
• Authors: Yu Zhang; Yujuan Yang; Jing Guo; Limei Cui; Liping Yang; Yumei Li; Yakui Mou; Chuanliang Jia; Luo Zhang; Xicheng Song
• Keywords: Allergic Inflammation; Regulatory T cell

MicroRNA (miR)-146a, as an important immune regulatory factor with anti-inflammatory effect, plays a crucial role in T-cell (Tregs) differentiation and function allergic rhinitis (AR). The present study aimed to investigate the mechanism employed by miR-146a control Treg AR.Expression of STAT5b peripheral blood mononuclear cells (PBMCs) nasal mucosa from patients AR was detected qPCR Western blotting. Tregs were quantified flow cytometry knockdown or PBMCs. FOXP3, IL-10, TGF-β levels blotting ELISA overexpressing PBMCs, well PBMCs miR-146a. effect on observed luciferase assay experiments.Levels miR146a significantly lower group than group. There fewer compared Expression decreased but increased overexpression In contrast, these factors, inhibited this effect. Luciferase experiments showed that bound directly STAT5b.miR-146a enhances positively targeting STAT5b.