Effects of glycerol, equilibration time and antioxidants on post-thaw functional integrity of bovine spermatozoa directly obtained from epididymis

• DOI: 10.1111/and.12623
• Publication Date: 2016-06-02T09:43:09Z
• Authors: F. C. Almeida; S. V. Silva; H. M. Souza; W. A. Gomes; J. A. C. Lima Filho; A. A. Wicke; A. M. Batista; M. M. P. Guerra
• Keywords: Yolk

This work aimed to evaluate the effect of stabilisation times, glycerol concentration, and catalase superoxide dismutase supplementation diluent on parameters frozen-thawed spermatozoa from epididymis Nelore bulls: Experiment 1: diluted in Tris-egg yolk with (3%, 5% or 7%) times (0, 2 4 hr at 5°C); 2: only, (CAT, 50 100 U ml−1) (SOD, ml−1). Frozen-thawed were evaluated for kinetic parameters, plasma membrane acrosome integrity, mitochondrial activity IVF capacity. ALH BCF affected (p < .05) by 3% after 4-hr equilibration time 7% 2-hr time. Glycerol had lower iPM iAc hr. greater hPMM than 0 SOD ml−1 linearity wobble compared control group. No was observed differences fertilisation rate among groups. In conclusion, extender is suitable preservation sperm kinetics integrity. CAT (50 (50–100 no beneficial effects kinetics, acrosomal capacity bulls.