Comparative analysis of endometrial, vaginal, and gut microbiota in patients with and without adenomyosis

• DOI: 10.1111/aogs.14847
• Publication Date: 2024-04-25T11:53:58Z
• Authors: Marta Valdés‐Bango; Meritxell Gracia; Elisa Rubio; Andrea Vergara; Climent Casals‐Pa...; Cristina Ros; Mariona Rius; Maria Ángeles Mar...; Eduard Mension; Lara Quintas; Francisco Carmona
• Keywords: Adenomyosis

Abstract Introduction Alterations in microbiota composition have been implicated a variety of human diseases. Patients with adenomyosis present immune dysregulation leading to persistent chronic inflammatory response. In this context, the hypothesis that alterations may be involved pathogenesis adenomyosis, by affecting epigenetic, immunologic, and biochemical functions host, has recently postulated. The aim study was compare vagina, endometrium, gut individuals without adenomyosis. Material Methods Cross‐sectional including 38 patients 46 controls, performed between September 2021 October 2022 university hospital‐based research center. diagnosis based on sonographic criteria. Fecal, vaginal, endometrial samples were collected. Study using 16S rRNA gene sequencing. Results exhibited significant reduction microbial alpha diversity compared healthy controls (Chao1 p = 0.012, Fisher 0.005, Observed species 0.005). Beta‐diversity analysis showed differences compositions both vaginal control group (Adonis ‐value 0.001; R 2 0.03 Adonis 0.034; 0.04 respectively). Specific bacterial taxa found either overrepresented (Rhodospirillales, Ruminococcus gauvreauii group, Ruminococcaceae, Actinomyces) or underrepresented controls. Distinct profiles identified among internal external phenotypes. Conclusions revealed reduced patients, accompanied distinct Overrepresented noted variations those These findings suggest potential association indicating need for further comprehensively understand implications these differences.