Chronic glucocorticoid treatment enhances lipogenic activity in visceral adipocytes of male Wistar rats

• DOI: 10.1111/apha.12226
• Publication Date: 2014-01-11T18:55:27Z
• Authors: P. Chimin; T. da S. M. Farias; F. L. Torres‐Leal; A. Bolsoni‐Lopes; A. B. Campaña; S. Andreotti; F. B. Lima
• Keywords: Lipogenesis; Malic enzyme; Fatty acid synthesis

Glucocorticoid (GC) in excess promotes the redistribution of adipose tissue from peripheral to central sites body. In this study, we characterized an experimental condition prolonged GC and investigated its effect on lipogenic metabolism white tissue.Twenty male Wistar rats were divided into control (CON) dexamethasone-treated (DEX) groups. DEX group received dexamethasone (0.25 mg kg(-1) day(-1) ) during 4 weeks, while CON saline. Animals killed subcutaneous (SC), retroperitoneal (RP) mesenteric (MS) fat pads excised, weighed processed for adipocyte isolation, morphometric cell analysis incorporation glucose lipids.The treatment effectively blocked hypothalamic-pituitary-adrenal axis, as verified by a 58% decrease plasma corticosterone levels 19% atrophy adrenal glands group. presented insulin resistance, intolerance, dyslipidaemia increased leptin hypertrophied adipocytes. They showed lipogenesis RP MS depots, with fatty acids triacylglycerol. Increased activity enzymes ATP-citrate lyase, acid synthase, glucose-6-phosphate dehydrogenase malic was only seen depot group, gene expression these enhanced SC depots.The adaptations promoted seemed be mainly due that supply NADPH required than increase more directly deal synthesis itself.