Reducing bacterial density in the semen of rainbow trout (Oncorhynchus mykiss) by applying gradient centrifugation and swim-up washing methods

• DOI: 10.1111/are.12835
• Publication Date: 2015-07-04T06:26:44Z
• Authors: Menekşe Didem Ercan; Aygül Ekici
• Keywords: Differential centrifugation

The objective of this study was to demonstrate the effectiveness disinfection procedures reduce bacterial load rainbow trout, Oncorhynchus mykiss (Walbaum 1792), semen. Fresh semen obtained from 3–4-year-old male species by abdominal sampling sperm into pre-cooled test tubes. After cryopreservation and thawing, experiments were accomplished at 4–9°C. 'Swim-up' gradient centrifugation used as a washing method with commercial kits. Phosphate buffered saline also solution. Bacterial growth tests employed before after samples. Samples inoculated on tryptic soy agar (TSA), modified Anacker Ordal (MAOA) well brain heart infusion (BHI) agar. using 'swim-up' for semen, many colonies observed. However, centrifugation, lower observed TSA, MAOA BHI. Some motile (40%) spermatozoa doing procedure. Although motility not satisfactory, apparently reduced contamination known mammalians.