Naloxone increases maturation rate and ratio of inner cell mass to total cells in blastocysts in pigs

• DOI: 10.1111/asj.12071
• Publication Date: 2013-05-03T09:44:05Z
• Authors: Thanh Quang Dang‐...; Nguyen Viet Linh; Rosa Minoia; Masahiro Kaneda; Tamas Somfai; Seiki Haraguchi; Satoshi Akagi; Kazuhiro Kikuchi; Michiko Nakai; Atsushi Tajima; Takashi Nagai
• Keywords: Germinal vesicle; In vitro maturation; Inner cell mass

Abstract The purposes of the present study were to examine effect naloxone, a mu‐opioid receptor ( MOR ) antagonist, on porcine oocyte maturation and embryo development. gene was expressed in germinal vesicle GV metaphase II M ‐ oocytes, one‐, four‐cell stage embryos blastocysts. In blastocysts, mainly inner cell mass ICM cells. Supplementation 10 −8 mol/L naloxone vitro IVM medium increased rate P < 0.05). However, −4 reduced 0.05) compared with control. presence during had no effects fertilization status subsequent embryonic development after culture IVC ). addition −3 dibutyryl cyclic adenosine monophosphate dbcAMP ), together into nuclear either or alone. did not improve at concentrations −6 mol/L, ratio total cells blastocysts conclusion, low concentration, increases Naloxone cAMP have synergistic maturation.