Effects of an Antimutagenic 1,4‐Dihydropyridine AV‐153 on Expression of Nitric Oxide Synthases and DNA Repair‐related Enzymes and Genes in Kidneys of Rats with a Streptozotocin Model of Diabetes Mellitus

• DOI: 10.1111/bcpt.12617
• Publication Date: 2016-05-10T14:52:46Z
• Authors: Kristīne Ošiņa; Evita Rostoka; Sergejs Isajevs; Jelizaveta Sokolo...; Tatjana Sjakste; Nikolajs Sjakste
• Keywords: Male; 0301 basic medicine; Dihydropyridines; DNA Repair; Nitric Oxide Synthase Type III; Reverse Transcriptase Polymerase Chain Reaction; Poly (ADP-Ribose) Polymerase-1; Gene Expression; Nitric Oxide Synthase Type II; Antimutagenic Agents; Kidney; Phosphoproteins; Niacin; Streptozocin; Diabetes Mellitus, Experimental; Rats; Histones; 03 medical and health sciences; Animals; RNA, Messenger; Rats, Wistar

Development of complications diabetes mellitus (DM), including diabetic nephropathy, is a complex multi-stage process, dependent on many factors the modification nitric oxide (NO) production and an impaired DNA repair. The goal this work was to study in vivo effects 1,4-dihydropyridine AV-153, known as antimutagen binder, expression several genes proteins involved NO metabolism repair kidneys rats with streptozotocin (STZ)-induced model DM. Transcription intensity monitored by means real-time RT-PCR immunohistochemistry. DM significantly induced PARP1 protein expression, while AV-153 (0.5 mg/kg) administration decreased it. increased Parp1 gene both intact animals. Expression H2afx mRNA γH2AX histone protein, marker breakage, not changed animals, but up-regulated without any impact expression. followed significant increase iNOS enzyme down-regulated up normal levels. iNos also found be unlike enhanced administration. eNOS eNos down-regulated, normalized level. compound animals appear beneficial, trend for normalization synthases observed.