Gambogic Acid Inhibits Melanoma through Regulation of miR‐199a‐3p/ZEB1 Signalling

• DOI: 10.1111/bcpt.13090
• Publication Date: 2018-06-30T13:54:31Z
• Authors: Lili Liang; Zhixin Zhang; Xiaowei Qin; Ying Gao; Peng Zhao; Jing Liu; Weihui Zeng
• Keywords: Gambogic acid; Viability assay

Malignant melanoma is an aggressive form of cancer which highly resistant to chemotherapy. We have previously found that gambogic acid (GA), a kind polyprenylated xanthone, exhibits antitumour role in melanoma. The study was designed investigate novel mechanisms the effect GA cells and implanted nude mice. Gambogic significantly decreased cell viability, increased apoptosis reduced migration invasion A375 cells. In addition, cisplatin-induced cytotoxicity both A375/CDDP by GA. expression miR-199a-3p tumours, inhibition prevented GA-induced on apoptosis, migration, cisplatin sensitivity mimics tumour weight volume vivo vitro. tissues cells, as compared with their controls. possessed potential binding site 3'-UTR zinc finger E-box homeobox (ZEB1). ZEB1 negatively correlated tissues. up-regulation sensitivity. Down-regulation identified important roles elucidated suppressor function through ZEB1. results highlight importance miR-199a-3p-ZEB1 signalling malignant provide targets for chemotherapy