Alterations in perivascular innervation function in mesenteric arteries from offspring of diabetic rats

• DOI: 10.1111/bph.13244
• Publication Date: 2015-07-14T16:43:44Z
• Authors: D B de Queiroz; E Sastre; L Caracuel; M Callejo; F E Xavier; J Blanco‐Rivero; G Balfagón
• Keywords: Vasomotor; Mesenteric arteries; Phentolamine; Sympathetic nervous system

We have reported that exposure to a diabetic intrauterine environment during pregnancy increases blood pressure in adult offspring, but the mechanisms involved are not completely understood. This study was designed analyse possible role of perivascular sympathetic and nitrergic innervation superior mesenteric artery (SMA) this effect.Diabetes induced pregnant Wistar rats by single injection streptozotocin. Endothelium-denuded vascular rings from offspring control (O-CR) (O-DR) were used. Vasomotor responses electrical field stimulation (EFS), NA NO donor DEA-NO studied. The expressions neuronal NOS (nNOS) phospho-nNOS (P-nNOS) release NA, ATP determined. Sympathetic nerve densities analysed immunofluorescence.Blood higher O-DR animals. EFS-induced vasoconstriction greater response decreased phentolamine more animals than their controls. L-NAME increased strongly O-CR segments. or modified. segments O-DR. nNOS expression modified, whereas P-nNOS similar both experimental groups.The activity is SMA net effect an increase contractions these These effects may contribute observed rats.