B3GNT3 expression suppresses cell migration and invasion and predicts favorable outcomes in neuroblastoma

• DOI: 10.1111/cas.12294
• Publication Date: 2013-10-12T01:36:28Z
• Authors: Wan‐Ling Ho; Mei‐Ieng Che; Chih‐Hsing Chou; Hsiu‐Hao Chang; Yung‐Ming Jeng; Wen‐Ming Hsu; Kai‐Hsin Lin; Min‐Chuan Huang
• Keywords: Paxillin

Aberrant expression of the simple mucin-type carbohydrate antigens such as T, Tn, sialyl-T and sialyl-Tn is associated with poor prognosis in several cancers. β1,3-N-acetylglucosaminyltransferase-3 (B3GNT3), a member β3GlcNAcT family, responsible for forming extended core 1 (T antigen) oligosaccharides. The role B3GNT3, which expressed various tissues including human fetal brain, regulating neuroblastoma (NB) formation cell behaviors remains unclear. Here, we showed that increased B3GNT3 evaluated using immunohistochemistry NB tumor correlated well histological grade differentiation favorable Shimada's subset pathology. Univariate multivariate analyses revealed positive predicted patients independent other prognostic markers. overexpression suppresses T antigen malignant phenotypes migration invasion SK-N-SH cells, whereas knockdown enhances these cells. Moreover, decreased phosphorylation focal adhesion kinase (FAK), Src, paxillin, Akt ERK1/2. We conclude predicts cancer behavior by modulating O-glycosylation signaling