Suppression of KIF3A inhibits triple negative breast cancer growth and metastasis by repressing Rb‐E2F signaling and epithelial‐mesenchymal transition

• DOI: 10.1111/cas.14324
• Publication Date: 2020-02-03T11:35:18Z
• Authors: Weilin Wang; Runze Zhang; Xiao Wang; Ning Wang; Jing Zhao; Zhimin Wei; Fenggang Xiang; Chengqin Wang
• Keywords: Triple-negative breast cancer

Triple negative breast cancer (TNBC) displays higher heterogeneity, stronger invasiveness, risk of metastasis and poorer prognosis compared with major subtypes. KIF3A, a member the kinesin family motor proteins, serves as microtubule-directed subunit has been found to regulate early development, ciliogenesis tumorigenesis. To explore expression, regulation mechanism KIF3A in TNBC, 3 TNBC cell lines, 98 cases primary paired adjacent tissues were examined. Immunohistochemistry, real-time PCR, western blot, flow cytometry, short hairpin RNA (shRNA) interference, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), colony formation techniques, transwell assays, scratch tests, xenograft mice models used. We that was overexpressed such high expression also associated tumor recurrence lymph node metastasis. Silencing suppressed proliferation by repressing Rb-E2F signaling pathway inhibited migration invasion epithelial-mesenchymal transition. The size smaller number lung metastatic nodules lower depletion MDA-MB-231 than control group. In addition, overexpression correlated chemoresistance. These results suggested progression