Suggested role for neutrophil extracellular trap formation in Ewing sarcoma immune microenvironment

• DOI: 10.1111/cas.15992
• Publication Date: 2023-11-02T05:52:57Z
• Authors: Rachel Shukrun; Szilvia Baron; Victoria Fidel; Anna Shusterman; Osnat Sher; Netanya Kollender; Dror Levin; Yair Peled; Yair Gortzak; Yoav Ben‐Shahar; Revital Caspi; Sagi Gordon; Michal Manisterski; Ronit Elhasid
• Keywords: Neutrophil Extracellular Traps

Abstract Ewing sarcoma (EWS) is a highly aggressive cancer with survival rate of 70%–80% for patients localized disease and under 30% those metastatic disease. Tumor‐infiltrating neutrophils (TIN) can generate extracellular net‐like DNA structures known as neutrophil traps (NETs). However, little about the presence prognostic significance tumor‐infiltrating NETs in EWS. Herein, we investigated 46 diagnosed EWS treated Tel Aviv Medical Center between 2010 2021. TINs were identified diagnostic biopsies by immunofluorescence. In addition, isolated from peripheral blood samples at diagnosis following neoadjuvant chemotherapy. The relationships NETs, pathological clinical features, outcomes analyzed. Our results demonstrate that TIN higher compared local High NET formation predicted poor response to chemotherapy, relapse, death ( p < 0.05). was significantly elevated among pediatric controls decreased conclusion, seems have role immune microenvironment. Their refine risk stratification, predict chemotherapy resistance survival, serve therapeutic target