Local and systemic effects of cat allergen nasal provocation

• DOI: 10.1111/cea.12434
• Publication Date: 2014-10-10T02:51:06Z
• Authors: G. W. Scadding; A. Eifan; M. Penagos; A. Dumitru; A. Switzer; O. McMahon; D. Phippard; A. Togias; S. R. Durham; M. H. Shamji
• Keywords: Tryptase; CD63; Nasal provocation test

Cat allergen is widely distributed in homes and schools; allergic sensitization common.To develop a model of cat nasal challenge to establish dose-response time-course characteristics investigate local systemic biomarkers inflammation.Nineteen cat-allergic individuals underwent titrated challenge, range 0.243 14.6 μg/mL Fel d1, matched diluent-only provocation. Clinical response 8 h was assessed by symptom scores peak inspiratory flow (PNIF). Nasal fluid collected using polyurethane sponges analysed ImmunoCAP multiplex assays. Whole blood cytometry for basophil surface CD63, CD107a, CD203c carried out at baseline 6 post-challenge.A seen PNIF, maximal 10 000 BU/mL (4.87 d1), P < 0.0001 vs. diluent. tryptase elevated 5 min after (P 0.05 diluent); eotaxin, IL-4, -5, -9, -13 were increased TSLP undetectable; IL-10, IL-17A, IL-33 unchanged compared diluent challenge. IL-5 IL-13 correlated inversely with PNIF (IL-5, r = -0.79, 0.0001; IL-13, -0.60, 0.006). Surface expression CD63 CD107a greater than baseline, both the presence (both 0.05) absence (CD63, 0.01; vitro stimulation; no changes on day.Cat produces Th2-driven inflammatory responses has potential as surrogate outcome measure clinical trials.