A comprehensive analysis of the allergenicity and IgE epitopes of myosinogen allergens in Scylla paramamosain

• DOI: 10.1111/cea.13266
• Publication Date: 2018-09-06T07:48:35Z
• Authors: Yang Yang; Meng Jun Hu; Teng Chuan Jin; Yong Xia Zhang; Guang Yu Liu; Yu Bao Li; Ming Li zhang; Min Jie Cao; Wen Jin Su; Guang Ming Liu
• Keywords: Scylla paramamosain; Basophil activation; Conformational epitope

Scylla paramamosain is one of the most common and serious food allergens in Asia. Therefore, research on its prevalence, accurate diagnosis, IgE-binding pattern crucial.To identify IgE epitopes myosinogen S. using phage peptide library.The prevalence allergy to crabs (AC) sensitization was analysed a questionnaire serological assay. BAT performed by flow cytometry, diagnostic performance evaluated relation purified from crab myosinogen. were identified display patients with AC. Sequence- structure-based bioinformatics analyses allergenic epitopes.Crab cause allergies this study. Subjects AC (n = 30) clear clinical symptoms immunoblotting BAT. All triggered basophil activation; surface expression CD63 CD203c higher allergic AK FLN c than SCP TIM. In addition six conformational SCP, linear eight identified. Five three TIM, nine ten also identified, sequence VH(I/T) L appeared both TIM c. The number showed consistency value BAT.BAT can be used for diagnosis Identification particular motifs could valuable tool prevention, treatment allergies.