Potential cross-reactivity of monoclonal antibodies against clinically relevant mycobacteria

• DOI: 10.1111/cei.12309
• Publication Date: 2014-02-28T13:07:42Z
• Authors: K Flores-Moreno; J S Celis-Meneses; D M Meneses-Ruiz; A I Castillo-Rodal; P Orduña; B A Montiel; Y López-Vidal
• Keywords: 0301 basic medicine; Mycobacterium Infections; Molecular Sequence Data; Antibodies, Monoclonal; Cross Reactions; Antibodies, Bacterial; Mass Spectrometry; Mycobacterium; 3. Good health; Epitopes; Mice; 03 medical and health sciences; Bacterial Proteins; Antibody Specificity; BCG Vaccine; Animals; Humans; Female; Amino Acid Sequence; Sequence Alignment; Mycobacterium avium

Tuberculosis is a disease caused by the Mycobacterium tuberculosis complex (MTb). In 2011, global mortality due to was 1·4 million individuals. The only available vaccine attenuated M. bovis [bacillus Calmette-Guérin (BCG)] strain, which confers variable protection against pulmonary tuberculosis. Some widely distributed non-tuberculous mycobacteria (NTM), such as avium and arupense, are also potential pathogens for humans. This work aimed produce characterize monoclonal antibodies BCG Mexico strain of MTb, subs. hominissuis arupense from NTM. Hybridomas were produced splenocytes BALB/c female mice immunized with radiation-inactivated mycobacteria, immunoglobulin (Ig)G2a antibody-producing clones highest antigenic recognition selected. selected clones, Mbv 2A10 Mexico, Mav 3H1 Mar 2D10 used in further studies. Enzyme-linked immunosorbent assay (ELISA) immune proteomics analyses characterized having cross-reactivity mycobacteria. Using mass spectrometry, number proteins recognized antibody (mAb) identified. These had roles metabolic processes, hypoxia, cell cycle dormancy. addition, Clustal W Immune Epitope Database (IEDB) in-silico analysis performed protein sequences that result conserved regions within probability epitopes could be Mbv2A10 Mav3H1 clones.