Higher levels of interleukin IL-17 and antigen-specific IL-17 responses in pulmonary sarcoidosis patients with Löfgren's syndrome

• DOI: 10.1111/cei.12403
• Publication Date: 2014-06-24T20:53:58Z
• Authors: M Ostadkarampour; A Eklund; D Moller; P Glader; C Olgart Höglund; A Lindén; J Grunewald; J Wahlström
• Keywords: Pulmonary sarcoidosis

Summary Sarcoidosis is a granulomatous disorder of unknown aetiology. The presence Mycobacterium tuberculosis catalase-peroxidase (mKatG) in sarcoidosis tissue has been reported. T helper type 1 (Th1) responses against mKatG have previously observed. However, little known about interleukin (IL)-17 and Th17 sarcoidosis. Here, we investigated the levels IL-17 frequencies IL-17-producing cells responding to patients with different prognosis. Peripheral blood bronchoalveolar lavage (BAL) were obtained from or without Löfgren's syndrome (often associated spontaneous recovery), also stratified according human leucocyte antigen (HLA) type. Cells producing interferon (IFN)-γ after stimulation enumerated by enzyme-linked immunospot (ELISPOT). level BAL fluid healthy controls was measured quantitative immuno-polymerase chain reaction (qIPCR). We performed flow cytometry immunohistochemistry for further characterization expression. Patients had higher frequency compared (P < 0·05). HLA-DR3+ (known particularly good prognosis) clearly 0·01) 0·05), respectively. No such difference between patient groups observed regard IFN-γ not either cytokine peripheral blood. These findings suggest that may be useful biomarker prognosis play role recovery typical syndrome.