Commensal microbiota stimulate systemic neutrophil migration through induction of Serum amyloid A

• DOI: 10.1111/cmi.12257
• Publication Date: 2013-12-26T08:52:42Z
• Authors: Michelle Kanther; Sarah Tomkovich; Sun Xiaolun; Melinda R. Grosser; Jaseol Koo; Edward J. Flynn; Christian Jobin; John F. Rawls
• Keywords: Transcriptional Activation; Serum Amyloid A Protein; Wound Healing; 0303 health sciences; Neutrophils; Microbiota; NF-kappa B; Gene Expression; Zebrafish Proteins; Immunity, Innate; Cell Line; 3. Good health; Mice, Inbred C57BL; Chemotaxis, Leukocyte; 03 medical and health sciences; Organ Specificity; Animals; Inflammation Mediators; Biomarkers; Zebrafish; Signal Transduction

Neutrophils serve critical roles in inflammatory responses to infection and injury, mechanisms governing their activity represent attractive targets for controlling inflammation. The commensal microbiota is known regulate the of neutrophils other leucocytes intestine, but systemic impact on remains unknown. Here we utilized vivo imaging gnotobiotic zebrafish reveal diverse effects colonization neutrophil development function. presence a resulted increased number myeloperoxidase expression, altered localization migratory behaviours. These homeostasis were accompanied by an recruitment injury. Genetic analysis identified microbiota-induced acute phase protein serum amyloid A (Saa) as host factor mediating microbial stimulation tissue-specific In vitro studies revealed that cells respond Saa exposure activating NF-κB, Saa-dependent migration requires NF-κB-dependent gene expression. results implicate important environmental regulating aspects function, role Saa-NF-κB signalling axis responses.