Expression and metabolism profiles of CVT associated with inflammatory responses and oxygen carrier ability in the brain

• DOI: 10.1111/cns.14494
• Publication Date: 2023-10-30T11:56:46Z
• Authors: Ling Kui; Yinming Jiao; Huimin Jiang; Guoyun Wang; Zongyu Li; Xunming Ji; Chen Zhou
• Keywords: Oxygen metabolism

Abstract Aim As the main type of stroke, incidence cerebral venous thrombosis (CVT) has been rising. However, comprehensive mechanisms behind it remain unclear. Thus, multi‐omics study is required to investigate mechanism after CVT and elucidate characteristic pathology stroke arterial stroke. Methods Adult rats were subjected MCAO models. Whole‐transcriptome sequencing (RNA‐seq) untargeted metabolomics analysis performed construct transcriptome metabolism profiles rat brains also MCAO. The difference analysis, functional annotation, enrichment performed. Results Through RNA‐seq differentially expressed genes (DEGs) screened. 174 specific including Il1a , Ccl9 Cxxl6 Tnfrsf14 etc., detected. hemoglobin genes, both Hba Hbb significantly downregulated CVT, compared Sham groups. Metabolism showed that had higher heterogeneity Metabolites N‐stearoyltyrosine, 5‐methoxy‐3‐indoleaceate, Afegostat, pipecolic acid, etc. specially regulated in CVT. immune infiltration was found a response, with abundance certain types cells increased, especially T helper cells. It important find prevalence activation inflammatory chemokine, cytokine, NOD‐like pathway, neutrophil extracellular trap. Conclusion We explored analyzed gene expression metabolomic characteristics revealed reaction markers levels. points out direction for early diagnosis treatment.