Linc02349 promotes osteogenesis of human umbilical cord‐derived stem cells by acting as a competing endogenous RNA for miR‐25‐3p and miR‐33b‐5p

• DOI: 10.1111/cpr.12814
• Publication Date: 2020-04-29T07:43:56Z
• Authors: Lihua Cao; Wei Liu; Yancheng Zhong; Yanru Zhang; Dan Gao; Tiantian He; Ying Liu; Zi Zou; Yuqing Mo; Shuping Peng; Cijun Shuai
• Keywords: STAT3 Transcription Factor; 0301 basic medicine; Osteoblasts; Transcription, Genetic; Cell Differentiation; Mesenchymal Stem Cells; Original Articles; Umbilical Cord; MicroRNAs; 03 medical and health sciences; HEK293 Cells; Osteogenesis; Humans; RNA, Long Noncoding; Promoter Regions, Genetic; Cells, Cultured

Increasing evidences suggest that inducing mesenchymal stem cells to differentiate into osteoblasts has been as an especially important component in the prevention and therapy for degenerative bone disease. Here, we identify a novel lncRNA, linc02349, which increases significantly during osteogenic differentiation.Human umbilical cord-derived (hUC-MSCs) dental pulp were used. Overexpression knockdown of linc02349 cell lines generated using lentiviral-mediated gene delivery method. Bioinformatics prediction, Ago2-RIP assay dual-luciferase reporter system employed examine miRNA interacts with linc02349. The RNA FISH was performed subcelluar location Alizarin Red S staining, ALP staining qPCR applied differentiation. potential linc02349-regulated genes, miR-25-3p miR-33b-5p, explored by ChIP, RIP Western blotting assays. Micro-CT used measure content formation vivo.Linc02349 overexpression improves differentiation vitro vivo analysis. Mechanistically, acts molecular sponge miR-33b-5p control expression abundance SMAD5 Wnt10b, respectively, eventually activated Dlx5/OSX pathway hence promoted In addition, revealed STAT3 promoter region positively regulates transcriptional activity.These findings modulates through acting regulating proposed new interaction between could be target process osteogenesis hUC-MSCs future clinical application.