Mast cell phenotypes in the allograft after lung transplantation

• DOI: 10.1111/ctr.12758
• Publication Date: 2016-05-05T09:57:30Z
• Authors: Amit Banga; Yingchun Han; Xiaofeng Wang; Fred H. Hsieh
• Keywords: Tryptase; Chymase; Parenchyma

The burden of mast cell (MC) infiltration and their phenotypes, MC-tryptase (MCT ) MC-tryptase/chymase (MCTC ), after lung transplantation (LT) has not been evaluated in human studies.We reviewed 20 transbronchial biopsy (TBLB) specimen from patients with early normal allograft (<6 months post-LT, n=5), late (>6 months, A2 or worse acute cellular rejection (ACR, chronic dysfunction (CLAD, n=5). Slides were immunostained for tryptase chymase. Total MC, MCT , MCTC to-MCT ratio compared between the four groups using a generalized linear mixed model.Irrespective clinicopathologic diagnosis, MC tends to increase time (r(2) =.56, P=.009). phenotype was significantly increased CLAD group (8.2±4.9 cells per HPF) comparison other three (early normal: 1.6±1.7, P=.0026; 2.5±2.3, P=.048; ACR: 2.7±3.5, P=.021). Further, as (P<.001 all comparisons).The may function time. Patients have an relative absolute MC. Future studies are needed confirm these findings evaluate potential pathologic role dysfunction.