In vivo wide‐field voltage imaging in zebrafish with voltage‐sensitive dye and genetically encoded voltage indicator

• DOI: 10.1111/dgd.12744
• Publication Date: 2021-08-19T18:48:25Z
• Authors: Kanae Hiyoshi; Asuka Shiraishi; Narumi Fukuda; Sachiko Tsuda
• Keywords: Channelrhodopsin; Biological neural network; Premovement neuronal activity; Neuromodulation

The brain consists of neural circuits, which are assemblies various neuron types. For understanding how the works, it is essential to identify functions each type and neuronal circuits. Recent advances in our function its development have been achieved using light detect activity. Optical measurement membrane potentials through voltage imaging a desirable approach, enabling fast, direct, simultaneous detection population neurons. Its high speed directness can help synaptic action hyperpolarization, encode critical information for function. Here, we describe vivo procedures that recently established zebrafish, powerful animal model developmental biology neuroscience. By applying two types sensors, voltage-sensitive dyes (VSDs, Di-4-ANEPPS) genetically encoded indicators (GEVIs, ASAP1), spatiotemporal dynamics signals be detected whole cerebellum spinal cord awake fish at single-cell levels. Combining this method with other approaches, such as optogenetics, behavioral analysis, electrophysiology would facilitate deeper network circuitry development.