SVEP1 plays a crucial role in epidermal differentiation

• DOI: 10.1111/exd.13256
• Publication Date: 2016-11-28T12:36:47Z
• Authors: Liat Samuelov; Qiaoli Li; Ron Bochner; Nicole A. Najor; Lauren Albrecht; Natalia Malchin; Tomer Goldsmith; Meital Grafi‐Cohen; Dan Vodo; Gilad Fainberg; Benjamin Meilik; Ilan Goldberg; Emily Warshauer; Tova Rogers; Sarah Edie; Akemi Ishida‐Yama...; Lisa Burzenski; Noam Erez; Steve A. Murray; Alan D. Irvine; Lenny Shultz; Kathleen J. Green; Jouni Uitto; Eli Sprecher; Ofer Sarig
• Keywords: Epidermis (zoology)

SVEP1 is a recently identified multidomain cell adhesion protein, homologous to the mouse polydom which has been shown mediate cell-cell in an integrin-dependent manner osteogenic cells. In this study, we characterized function epidermis. was found by qRT-PCR be ubiquitously expressed human tissues, including skin. Confocal microscopy revealed that normally mostly cytoplasm of basal and suprabasal epidermal Downregulation expression primary keratinocytes resulted decreased major differentiation markers. Similarly, downregulation associated with disturbed marked acanthosis three-dimensional skin equivalents. contrast, dispase assay failed demonstrate significant differences between expressing normal vs low levels SVEP1. Homozygous Svep1 knockout mice were embryonic lethal. Thus, assess importance for homoeostasis vivo, downregulated zebrafish embryos Svep1-specific splice morpholino. Scanning electron rugged epidermis perturbed microridge formation centre morphant larvae. Transmission analysis demonstrated abnormal disadhesion cells Svep1-deficient larvae compared controls. summary, our results indicate plays critical role during differentiation.