High calcium enhances the expression of double‐stranded RNA sensors and antiviral activity in epidermal keratinocytes

• DOI: 10.1111/exd.13456
• Publication Date: 2017-10-31T12:02:39Z
• Authors: Yuriko Yamamura; Shin Morizane; Takenobu Yamamoto; Jun Wada; Keiji Iwatsuki
• Keywords: Keratinocytes; 0303 health sciences; Interferon-Induced Helicase, IFIH1; beta-Defensins; Herpesvirus 1, Human; Viral Plaque Assay; Immunity, Innate; Toll-Like Receptor 3; Up-Regulation; 3. Good health; 03 medical and health sciences; Poly I-C; DEAD Box Protein 58; Humans; Calcium; Epidermis; Receptors, Immunologic; RNA, Double-Stranded; Signal Transduction; Skin

Double-stranded RNA (dsRNA) sensors including TLR3, MDA5 and RIG-I are expressed in epidermal keratinocytes play an important immunological role by enhancing various innate adaptive immune responses. Although the of elevated extracellular calcium concentration keratinocyte differentiation is well understood, effect high on dsRNA not studied. We investigated alterations sensor expression antiviral activity induced a keratinocytes. Normal human (NHEKs) were stimulated with and/or synthetic dsRNA, poly (I:C). IFIH1 (MDA5) DDX58 (RIG-I) measured via qPCR, IFN-β beta-defensin 2 (HBD2) levels using ELISA. TLR3 localization was evaluated immunocytofluorescence. Antiviral quantified virus plaque assays herpes simplex type 1 (HSV-1). High significantly upregulated mRNA NHEKs. In addition, enhanced (I:C)-induced anti-HSV-1 The molecule HBD2 but induction (I:C) calcium. Our findings indicate that enhance augment