Tanshinone IIA reduces palmitate‐induced apoptosis via inhibition of endoplasmic reticulum stress in HepG2 liver cells

• DOI: 10.1111/fcp.12510
• Publication Date: 2019-09-14T13:34:35Z
• Authors: Junjian Wang; Rui Hu; Chunyan Yin; Yanfeng Xiao
• Keywords: ATF6; Steatosis; Viability assay; Caspase 12

Research has indicated that stress on the endoplasmic reticulum (ER) of a cell affects pathogenesis metabolic disorders such as obesity, type 2 diabetes mellitus, and non-alcoholic fatty liver disease (NAFLD). Palmitate, saturated acid, is known to induce toxicity death in various types cells. Tanshinone IIA (Tan IIA), one effective components traditional Chinese medicine Danshen, was reported exhibit variety biochemical activities, including amelioration ER stress-mediated apoptosis renal preservation. To address hypothesis tan attenuates triglycerides (TG) accumulation via reducing stress, we studied effect experimentally induced using palmitate HepG2 Palmitate led cytotoxicity, TG accumulation, cells also strongly by increased GRP78, phosphorylation eIF2α, ATF6, CHOP. Pretreatment with (10 μm) significantly viability, decreased apoptotic death, reduced activity caspase-3. Meanwhile, palmitate-induced accumulation. Moreover, suppressed inhibited CHOP expression. In conclusion, protects exposed partially inhibiting excessive stress-induced apoptosis, hepatic steatosis. Therefore, therapeutic potential treatment NAFLD.