Endogenous hydrogen sulfide alleviates methotrexate‐induced cognitive impairment by attenuating endoplasmic reticulum stress‐induced apoptosis via CHOP and caspase‐12

• DOI: 10.1111/fcp.12543
• Publication Date: 2020-02-08T08:19:50Z
• Authors: Siyuan Lv; Ning Wu; Qiang Wang; Li‐Hua Yang
• Keywords: Male; 0303 health sciences; Doublecortin Protein; Apoptosis; Endoplasmic Reticulum Stress; Hippocampus; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Methotrexate; Animals; Cognitive Dysfunction; Hydrogen Sulfide; Maze Learning

Abstract The aim of this study was to estimate whether methotrexate (MTX) promotes cognitive impairment via increased ER stress and disrupted H 2 S signaling in the hippocampus may alleviate MTX‐induced by inhibiting through CHOP caspase‐12. Cognitive behaviors were observed Morris water maze test, apoptosis neurons assessed TUNEL assay. production analyzed DCX Ki67 immunohistochemistry. expressions caspase‐12 determined Western blot MTX expression number TUNEL‐positive cells endogenous S‐induced neuronal pyknosis hippocampal CA1 region. decreased DCX‐ Ki67‐positive DG results showed that could damage spatial memory rats. changes test mice levels serum hippocampus, as well caspase‐12‐positive indicated induced