MicroRNA‐15a promotes neuroblastoma migration by targeting reversion‐inducing cysteine‐rich protein with Kazal motifs (RECK) and regulating matrix metalloproteinase‐9 expression

• DOI: 10.1111/febs.12074
• Publication Date: 2012-11-23T17:46:08Z
• Authors: Chen Xin; Bao Buhe; Lu Hongting; Yang Chuanmin; Hao Xiwei; Zhang Hong; Han Lulu; Dong Qian; Wang Renjie
• Keywords: Reversion; Ectopic expression

In this study, we found that the expression of miR-15a was positively correlated with neuroblastoma (NB) clinical pathological stage and negatively reversion-inducing cysteine-rich protein Kazal motifs (RECK) expression. Using enhanced green fluorescent (EGFP) reporter construct carrying 3'-UTR RECK, identified RECK as a direct target miR-15a. Suppression significantly decreased migration ability GI-LA-N SK-N-SH cell lines, whereas overexpression increased ability; these effects could be partly reversed by inhibition or ectopic Moreover, secreted matrix metalloproteinase-9 in culture medium through regulating RECK. These findings provide new insights into characteristics miR-15a-RECK-matrix axis NB progression, especially invasion.