Isolation of Helicobacter pylori using leftover tissue in the rapid urease test kit

• DOI: 10.1111/hel.12733
• Publication Date: 2020-08-03T11:00:55Z
• Authors: Eun Jeong Gong; Ji Yong Ahn; Da Kyung Jung; Sun Mi Lee; Gyu Young Pih; Ga Hee Kim; Hee Kyong Na; Jeong Hoon Lee; Hwoon‐Yong Jung; Jung Mogg Kim
• Keywords: Rapid urease test; Gastric antrum; Isolation

Abstract Background and Aims Isolation of Helicobacter pylori is considered difficult because the requirement additional biopsy tissue effort involved in isolation bacterium. We investigated whether H can be cultured from samples used for rapid urease test (RUT). Methods Totally, 174 specimens 87 patients referred endoscopy were prospectively included. During endoscopy, two obtained, one each gastric antrum corpus, placed into a commercially available RUT kit. After detection activity, was using leftover RUT, regardless result. Results successfully isolated 72.4% (63/87) patients. In 32 patients, both specimens, while 31 it either or corpus. Eighty‐one strains 141 with positive results (57.4%), whereas 14 33 negative (42.4%). The median interval between acquisition inoculation onto media 3.6 hours (range: 0.5‐27.5 hours) cases successful cultures, compared to 23.5 0.5‐76.0 failed cultures. Among tissues, 80.4% (45/56) when inoculated within 4 biopsy. Conclusions kits as transport , this most efficient test.