Association between bacteria occurring in the apical canal system and expression of bone‐resorbing mediators and matrix metalloproteinases in apical periodontitis

• DOI: 10.1111/iej.12895
• Publication Date: 2018-01-24T07:42:38Z
• Authors: A. Takahama; I. N. Rôças; I. S. P. Faustino; F. R. F. Alves; R. S. Azevedo; C. C. Gomes; W. R. Araújo‐Filho; J. F. Siqueira
• Keywords: Pulp necrosis

Abstract Aim To evaluate the association between presence of selected bacterial species/groups in apical root canal and expression mediators soft bone tissue destruction periodontitis lesions. Relationships bacteria some other features were also investigated. Methodology Seventeen freshly extracted teeth with pulp necrosis included. The segment was sectioned cryopulverized; DNA evaluated for 9 using real‐time polymerase chain reaction. Lesions processed histopathological immunohistochemical analyses, which targeted matrix metalloproteinase‐2 ( MMP ‐2) ‐9 ‐9), receptor activator NF κB RANK ), ligand RANKL ) osteoprotegerin OPG ). Associations target these mediators, symptoms, lesion size diagnosis evaluated. Data analysed chi‐square, Fisher's exact, Mann–Whitney Pearson tests. P values lower than 0.05 considered significant. Results All pulverized samples positive bacteria. most prevalent taxa Actinobacteria (53%), Streptococcus species (35%), Fusobacterium Parvimonas micra (18%). exhibited a high mean lesions ‐2: 82%; ‐9: 73%; : 78%; ; 81%; 83%). Mean ratio significantly higher granulomas cysts < 0.05, test). associated granulomas, ‐2 expression, all, exact test or No significant associations found. Conclusion may play an important role active phase periodontitis.