Annexin V expression on CD4+ T cells with regulatory function

• DOI: 10.1111/imm.13140
• Publication Date: 2019-10-23T19:01:26Z
• Authors: Anna‐Lena Bollinger; Thomas Bollinger; Jan Rupp; Kensuke Shima; Natalie Gross; Laura Padayachy; Rachel Chicheport...; Gisella L. Puga Yung; Jörg Dieter Seebach
• Keywords: Expression (computer science)

Regulatory T (Treg) cells induce immunologic tolerance by suppressing effector functions of conventional lymphocytes in the periphery. On other hand, immune silencing is mediated recognition phosphatidylserine (PS) on apoptotic phagocytes. Here we describe expression PS-binding protein Annexin V (ANXA5) CD4+ CD25hi Treg at mRNA and levels. ANXA5+ constitute about 0·1%-0·6% peripheral blood CD3+ cells, exhibit co-expression several markers, such as Forkhead box P3, programmed cell death protein-1, cytotoxic T-lymphocyte antigen-4 CD38. In vitro, showed enhanced adhesion to PS+ endothelial cells. Stimulated anti-CD3 syngeneic antigen-presenting expanded absence exogenous interleukin-2. suppressed ANXA5- T-cell proliferation mammalian target rapamycin phosphorylation, partially dependent contact. T-cell-mediated suppression was allo-specific accompanied an increased production anti-inflammatory mediators. vivo, using a model delayed type hypersensitivity, murine inhibited helper 1 responses. conclusion, report for first time ANXA5 subset that might bridge classical regulatory function with silencing.