Effect of laparoscopy versus laparotomy on circulating tumor cells using isolation by size of epithelial tumor cells

• DOI: 10.1111/j.1048-891x.2004.014205.x
• Publication Date: 2004-04-16T11:51:32Z
• Authors: L. Lelievre; P. Paterlini-Brechot; S. Camatte; E. Tartour; M. Aggerbeck; F. Vilde; F. Lecuru
• Keywords: Ovarian Neoplasms; Laparotomy; Neoplastic Cells, Circulating; Rats; 3. Good health; Disease Models, Animal; Random Allocation; Rats, Nude; 03 medical and health sciences; 0302 clinical medicine; Animals; Female; Laparoscopy; Neoplasm Transplantation

To assess the effect of laparoscopy on circulating tumor cell (CTC) detection in case of carcinosis.We compared laparoscopy versus laparotomy on tumor cell blood release in an animal model of ovarian carcinosis obtained by intraperitoneal inoculation of IGR-OV1 cells in nude rats. Animals were randomly assigned to one of the following groups: CO(2) laparoscopy (L), gasless laparoscopy (GL), midline laparotomy (ML), or general anesthesia as control (C). A 0.5 ml blood sample was taken in each case before and after experiment and tested with a novel assay, ISET (isolation by size of epithelial tumor cells), which isolates CTC by filtration on account of their size. Statistics were performed with the Fisher's and the Chi-square tests.Ten rats were included in each group. We did not find any significant difference in CTC prevalence before and after surgery (2/14 versus 3/19, respectively, P = 1). Similarly, the three surgical accesses were equivalent with one post-experiment detection per group: 1/5 for L, 1/7 for ML, 1/7 for GL, and 1/6 for C (P = 0.9).This trial did not show any deleterious effect of laparoscopy on CTC when compared to laparotomy.