Use of Antiserum‐Coated Latex Particles for Serotyping Streptococcus pneumoniae

• DOI: 10.1111/j.1348-0421.2001.tb01284.x
• Publication Date: 2013-11-14T15:43:15Z
• Authors: Satoru Arai; Toshifumi Konda; Akihito Wada; Yasuko Matsunaga; Nobuhiko Okabe; Haruo Watanabe; Sakae Inouye
• Keywords: 0301 basic medicine; Antigens, Bacterial; Immune Sera; Penicillin G; Microbial Sensitivity Tests; Antibodies, Bacterial; Sensitivity and Specificity; Microspheres; 3. Good health; 03 medical and health sciences; Streptococcus pneumoniae; Immunoglobulin G; Animals; Humans; Rabbits; Serotyping; Staphylococcal Protein A; Latex Fixation Tests

AbstractThe Quellung reaction provides a standard means for serotyping Streptococcus pneumoniae, but it requires microscopic examination with skillful technique. We have developed an improved agglutination method with anti‐rabbit IgG‐coated latex particles, which are sensitized with pooled antisera for serotyping/serogrouping S. pneumoniae. Our method is as specific and sensitive as the Quellung test, and much easier to perform.