Contribution of Chromosome 9p21‐22 Deletion to the Progression of Human Renal Cell Carcinoma

• DOI: 10.1111/j.1349-7006.1995.tb03087.x
• Publication Date: 2005-08-24T08:14:23Z
• Authors: Hidefumi Kinoshita; Hitoshi Yamada; Osamu Ogawa; Yoshiyuki Kakehi; Mitsuhiko Osaka; Eijiro Nakamura; Mutsuki Mishina; Tomonori Habuchi; Rei Takahashi; Taketoshi Sugiyama; Osamu Yoshida
• Keywords: Southern blot

To investigate the possible role of genomic aberrations chromosome 9p21‐22 in tumorigenesis human renal cell carcinoma (RCC), 10 RCC lines, 55 primary RCCs and 5 metastatic lesions were studied by Southern blotting polymerase chain reaction‐based analysis. Nine lines showed a homozygous deletion MTS1/CDKN2/(p16) , while only 1 tumors had this deletion. Loss heterozygosity on was observed informative from patients with metastasis, but 4 31 (13%) without metastasis ( P = 0.025). Furthermore, 3 (60%) hemi‐ or MTS1/CDKN2 . These results indicate that may be relatively late event could associated metastasis.