Expression of Macrophage Migration Inhibitory Factor in Human Breast Cancer: Association with Nodal Spread

• DOI: 10.1111/j.1349-7006.2002.tb01269.x
• Publication Date: 2005-09-02T08:12:17Z
• Authors: Hiroko Bando; Gaku Matsumoto; Masaki Bando; Mariko Muta; Taeko Ogawa; Nobuaki Funata; Jun Nishihira; Morio Koike; Masakazu Toi
• Keywords: Adult; 0301 basic medicine; Neovascularization, Pathologic; Macrophages; Blotting, Western; Breast Neoplasms; Enzyme-Linked Immunosorbent Assay; Middle Aged; Prognosis; Immunohistochemistry; Article; 3. Good health; Immunoenzyme Techniques; 03 medical and health sciences; Phenotype; Lymphatic Metastasis; Humans; Female; Endothelium, Vascular; Neoplasm Metastasis; Macrophage Migration-Inhibitory Factors; Cells, Cultured; Aged; Interleukin-1

Macrophage migration inhibitory factor (MIF) is known to exert pleiotropic functions including inhibition of macrophage migration, anchoring, and counteraction the anti‐inflammatory immunosuppressive activity glucocorticoids. Ninety‐three primary breast cancer tissues 64 sera patients were analyzed for expression MIF. The clinico‐pathological significance MIF was evaluated. It found that frequently over‐expressed in tissues. RT‐PCR western blotting analysis confirmed wild‐type expressed, immunohistochemical showed localized at tumor cells as well stromal cells, tumor‐associated macrophages. Intra‐tumoral protein concentrations detected by enzyme‐linked immunosorbent assay (ELISA) varied with a median value 1821 ng/mg (range: 8–8126 protein), correlated inversely nodal involvement ( P =0.039). No significant correlation observed other factors size, menopausal status hormone receptors. circulating level ranged up 105.7 ng/ml (median: 17.3 ng/ml), it also correlate number involved nodes =0.02). A comparative study soluble inflammatory mediators intratumoral levels significantly associated those interleukin‐1β, suggesting interactions between macrophages play an important role up‐regulation multifunctional inflammatory/immune mediator expressed cancer, its spread. Thus, seems tumor‐stroma cancers, particularly phenotype node‐negative or minimal