Tumor regression by combined immunotherapy and hyperthermia using magnetic nanoparticles in an experimental subcutaneous murine melanoma

• DOI: 10.1111/j.1349-7006.2003.tb01438.x
• Publication Date: 2005-08-19T10:25:50Z
• Authors: Akira Ito; Kouji Tanaka; Kazuyoshi Kondo; Masashige Shinkai; Hiroyuki Honda; Kazuhiko Matsumoto; Toshiaki Saida; Takeshi Kobayashi
• Keywords: Time Factors; Survival; Melanoma, Experimental; Granulocyte-Macrophage Colony-Stimulating Factor; Hyperthermia, Induced; Combined Modality Therapy; Recombinant Proteins; 3. Good health; Mice, Inbred C57BL; Disease Models, Animal; Magnetics; Mice; 03 medical and health sciences; 0302 clinical medicine; Tumor Cells, Cultured; Animals; Interleukin-2; HSP70 Heat-Shock Proteins; Immunotherapy

Immunotherapy (IT) has become an accepted therapeutic modality. We previously reported that intracellular hyperthermia (IH) using magnetic nanoparticles induces antitumor immunity. undertook these studies in order to study the combined effects of IT and IH on melanoma. Magnetite cationic liposomes (MCLs) have a positive surface charge generate heat alternating field (AMF) due hysteresis loss. MCLs were injected into B16 melanoma nodule C57BL/6 mice, which subjected AMF for 30 min. The temperature at tumor reached 43°C was maintained by controlling intensity. At 24 h after IH, interleukin‐2 (IL‐2) or granulocyte macrophage‐colony stimulating factor (GM‐CSF) directly Mice divided six groups: group I (control), II (IH), III (IL‐2), IV (GM‐CSF), V (IH+IL‐2), VI (IH+GM‐CSF). Complete regression tumors observed mice groups (75% (6/ 8) 40% (4/10) respectively), while no other groups. This supports use patients with advanced malignancies. (Cancer Sci 2003; 94: 308–313)