Comparison of microsatellite and SNP DNA markers for pedigree assignment in Black Tiger shrimp,Penaeus monodon

• DOI: 10.1111/j.1365-2109.2012.03243.x
• Publication Date: 2012-08-03T17:53:38Z
• Authors: Melony J Sellars; Leanne Dierens; Sean McWilliam; Bryce Little; Brian Murphy; Greg J Coman; William Barendse; John Henshall
• Keywords: Penaeus monodon; SNP

Custom-built single-nucleotide-polymorphism (SNP) marker systems that are compatible with Sequenom® chemistry compared a general purpose microsatellite system in their ability to accurately assign Black Tiger shrimp parentage. The consisted of 13 markers, while the SNP comprised 63 (SNPa), 59 (SNPb) or 122 markers (SNPab). Comparisons were made using animals from commercial breeding lines thresholds for assignment derived simulated genotypes. Pedigree was highest when panel SNPab used. Panel SNPa, SNPb and Msat functioned an overall similar level power pedigree assignment, however, some families, not as powerful. diploid genotypes higher all panels Msat. Overall three provided parentage rates suitable programs greater than 96.8% correct assignments 99.3%. Compared panel, custom-built panels, whether they operate single combined have improved perform dam sire progeny provide faster turnaround time chemistry.