High expression of connective tissue growth factor in pre‐B acute lymphoblastic leukaemia

• DOI: 10.1111/j.1365-2141.2007.06739.x
• Publication Date: 2007-08-23T15:45:15Z
• Authors: Joanne M. Boag; Alex H. Beesley; Martin J. Firth; Joseph R. Freitas; Jette Ford; David R. Brigstock; Nicholas H. De Klerk; Ursula R. Kees
• Keywords: PDGFB

Summary In recent years microarrays have been used extensively to characterize gene expression in acute lymphoblastic leukaemia (ALL). Few studies, however, analysed normal haematopoietic cell populations identify altered ALL. We oligonucleotide compare the profile of paediatric precursor‐B (pre‐B) ALL specimens with two control populations, CD34 + and CD19 IgM − cells, focus on genes linked leukemogenesis. A set eight was identified a ninefold higher average compared cells. All these were significantly deregulated an independent cohort 101 specimens. One gene, connective tissue growth factor ( CTGF , also known as CCN2 ), had exceptionally high expression, which confirmed three studies. Further analysis revealed exclusive B‐lineage, not T‐lineage, Within B‐lineage approximately 75% consistently positive for containing E2A‐PBX1 translocation showed low or no expression. Protein studies using Western blot demonstrated presence cell‐conditioned media. These findings indicate that is secreted by pre‐B cells may play role pathophysiology this disease.