Insulin‐like growth factor‐I and transforming growth factor‐α stimulate differentiation of type A spermatogonia in organ culture of adult mouse cryptorchid testes

• DOI: 10.1111/j.1365-2605.1995.tb00928.x
• Publication Date: 2008-05-16T08:05:01Z
• Authors: Y. TAJIMA; D. WATANABE; U. KOSHIMIZU; T. MATSUZAWA; Y. NISHIMUNE
• Keywords: Organ culture

Summary This study assessed the effect of growth factors on testicular germ cell differentiation in vitro . Testicular fragments experimentally prepared cryptorchid testes adult mice were cultured for 9 days serum‐free media containing various concentrations IGF‐I, TGF‐α, FGF, and PDGF. Their histology was then examined under a light microscope. Each type mitotic seminiferous tubules counted per 1000 Sertoli cells. IGF‐I at concentration 10 ng/ml induced maximal A spermatogonia. TGF‐α ranging from 1 to also stimulated differentiation, whereas FGF PDGF did not show any stimulation spermatogonial this experimental system.