Tropomyosin, the Major Oyster Crassostrea gigas Allergen and its IgE‐binding Epitopes

• DOI: 10.1111/j.1365-2621.1998.tb15672.x
• Publication Date: 2006-07-20T19:00:06Z
• Authors: MASARU ISHIKAWA; MASAMI ISHIDA; KUNIYOSHI SHIMAKURA; YUJI NAGASHIMA; KAZUO SHIOMI
• Keywords: Tropomyosin

ABSTRACT The major allergen ( Cra g 1) isolated from the oyster Crassostrea gigas was digested with lysylendopeptidase. Twenty‐one peptide fragments were digest by reverse‐phase HPLC and some of them sequenced. 1 identified as tropomyosin. In competitive ELISA inhibition analyses lysylendopeptidase fragments, a (K21) showed maximum binding IgE antibodies in sensitive human serum to 1. On further tryptic hydrolysis K21, two peptides exhibited high inhibitory ability equivalent K21. These results suggested that had an IgE‐binding epitope segment 92–105 (IQLLEEDMERSEER) which dissimilar those (tropomyosin) shrimp Penaeus indicus.