Phenotypic properties, drug susceptibility and genetic relatedness of Stenotrophomonas maltophilia clinical strains from seven hospitals in Rio de Janeiro, Brazil

• DOI: 10.1111/j.1365-2672.2004.02248.x
• Publication Date: 2004-04-08T11:09:48Z
• Authors: L.H. Travassos; M.N. Pinheiro; F.S. Coelho; J.L.M. Sampaio; V.L.C. Merquior; E.A. Marques
• Keywords: Stenotrophomonas maltophilia; Ticarcillin; MacConkey agar; Housekeeping gene

To investigate phenotypic aspects including biotyping, drug susceptibility and production of extracellular enzymes genetic diversity Stenotrophomonas maltophilia clinical strains obtained from seven hospitals in Rio de Janeiro, Brazil.Thirty-nine S. were investigated by biotying, testing, detection randomly amplified polymorphic DNA (RAPD)-PCR. Biotyping distinguished 13 biotypes among 39, one them was prevalent. The majority the produced DNase, gelatinase haemolysin. Protease, lipases phospholipase C activities observed highly variable amounts. None elastase producer. percentage full susceptibility, agar dilution, 100, 94.8, 81.6 26.3% for trimethoprim/sulphametoxazole, ticarcillin/clavulanate, ciprofloxacin ceftazidime, respectively. Thirty-three RAPD-PCR profiles suggesting multiple sources acquisition.The results pointed out necessity monitoring especially critical hospital wards, to assure effective control measures.Despite strains, two situations it indistinguishable isolated different patients who had been hospitalized same ward, possibility nosocomial transmission that until now has rarely related.