Heat shock‐induced biphasic Ca2+ signature and OsCaM1‐1 nuclear localization mediate downstream signalling in acquisition of thermotolerance in rice (Oryza sativa L.)

• DOI: 10.1111/j.1365-3040.2012.02508.x
• Publication Date: 2012-03-19T15:20:58Z
• Authors: HUI‐CHEN WU; DAN‐LI LUO; FLORENCE VIGNOLS; TSUNG‐LUO JINN
• Keywords: Epicotyl

We investigated heat shock (HS)-triggered Ca(2+) signalling transduced by a sensor, calmodulin (CaM), linked to early transcriptome changes of HS-responsive genes in rice. observed biphasic [Ca(2+) ](cyt) signature root cells that was distinct from epicotyl and leaf cells, which showed monophasic response after HS. Treatment with A23187 generated an intense sustained increase Conversely, treatment chelator, L-type channel blocker CaM antagonist, but not intracellular release inhibitor, strongly inhibited the increased . HS combined accelerated expression OsCaM1-1 sHSPC/N genes, suggests HS-induced apoplastic influx is responsible for downstream signalling. In addition, nucleus followed signature, may provide information necessary direct HS-related gene expression. Overexpression induced /HS-related AtCBK3, AtPP7, AtHSF AtHSP at non-inducing temperature enhanced intrinsic thermotolerance transgenic Arabidopsis. Therefore, HS-triggered rapid increases , together its nuclear localization, are important mediating acquisition