Activation of a COI1‐dependent pathway in Arabidopsis by Pseudomonas syringae type III effectors and coronatine

• DOI: 10.1111/j.1365-313x.2003.01986.x
• Publication Date: 2004-12-29T18:49:56Z
• Authors: Ping He; Satya Chintamanani; Zhongying Chen; Lihuang Zhu; Barbara N. Kunkel; James R. Alfano; Xiaoyan Tang; Jian‐Min Zhou
• Keywords: Coronatine; Pseudomonas syringae; Phytotoxin; Virulence factor

Summary Gram‐negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell‐wall‐degrading enzymes to promote parasitism in plants. However, little is known about how these alter plant cellular responses disease. In this study, we show that virulent Pseudomonas syringae strains activate transcription an Arabidopsis ethylene response factor ( ERF ) gene, RAP2.6 , coronatine insensitive 1 (COI1)‐dependent manner. A highly sensitive promoter‐firefly luciferase RAP2.6‐LUC reporter line was developed monitor activities various bacterial genes. Analyses P. pv. tomato DC3000 mutants indicated both system phytotoxin are required for induction. We at least five individual effectors, avirulence B (AvrB), AvrRpt2, AvrPphB, HopPtoK, AvrPphE Pto contributed Gene‐for‐gene recognition not involved induction because plants lacking RPM1 RPS2 responded normally AvrB AvrRpt2 expression. Interestingly, role can be partially substituted addition avrB DC3000, suggesting may mimic coronatine. These results suggest act augmenting COI1‐dependent pathway parasitism.